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. 2018 Aug 13;8:12042. doi: 10.1038/s41598-018-30525-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The granzymes concentration [pg/ml] in supernatants collected during the culture of stimulated DCs (n = 8–10). Data presented as fold change over the level obtained from iDCs culture. Lysates and TCM used in these analyses were collected from cultures of HCT116 and HT29 CRC lines expanded in adherent or spherical forms (allogenic), and CRC patients-derived CSCs (autologous). Bars represent median value (*p < 0.05 vs iDCs, ^#p < 0.05 vs LPS; ANOVA Kruskal – Wallis test, n = 15).