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. 2018 Aug 13;8:12042. doi: 10.1038/s41598-018-30525-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The differences in the proportion of T regulatory-like CD4⁺CD25⁺ cells after 24 h co-culture with stimulated DCs. Lymphocytes were measured amongst the effector cells acquired during the isolation of monocytic precursors for DCs expansion. Lysates and TCM used in these analyses were collected from cultures of HCT116 and HT29 cell lines expanded in adherent or spherical forms (allogenic). Data presented as MFI fold change over the level obtained after spontaneous proliferation of the control CD4⁺CD25⁺ T cells. The control value was set at the level of 1. Bars and whiskers represent mean ± SEM (*p < 0.05 vs iDCs, ^#p < 0.05 vs LPS; ANOVA Kruskal – Wallis test, n = 15).