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. 2018 Aug 13;8:12056. doi: 10.1038/s41598-018-30561-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The change in DJ-1 oxidation in the substantia nigra (SN) of MPTP-treated mice. After the administration of PBS and MPTP (15 mg/kg, 3 times i.p.), each brain was separated and subjected to analysis. (a) Striatal levels of dopamine in mice were determined 3 days after MPTP administration (n = 5). **P < 0.01, Student’s t-test. (b) Protein lysates of the SN of MPTP-treated mice were subjected to western blot analyses using each specific Ab. The relative band densities of oxDJ-1 relative to DJ-1 and tyrosine hydroxylase (TH) relative to GAPDH were calculated and are presented as mean ± SD (n = 4). **P < 0.01, *P < 0.05, Tukey-Kramer test, ANOVA, when compared with day 0. (c) Modification of DJ-1 in the SN of MPTP-treated mice was evaluated using 2D-PAGE and western blotting for DJ-1. The filled triangles and open triangles indicate native and oxidized DJ-1, respectively. The striped triangles A and B indicate DJ-1 with an unknown modification. The relative densities of each spot were estimated. Graphs display the relative densities of each spot compared with PBS control as mean ± SD (n = 5). **P < 0.01, Student’s t-test.