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. 2018 Aug 13;8:12013. doi: 10.1038/s41598-018-30508-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of ORF1119 PvGal-ase activity toward fission yeast galactomannan. (A) Level of extracellular negatively charged glycans. S. pombe WT strain in the presence or absence of recombinant ORF1119 PvGal-ase was stained with alcian blue. S. pombe pvg1Δ strain was used as a negative control. (B) Time course of the reduction in alcian blue staining of S. pombe WT strain incubated with recombinant ORF1119 PvGal-ase.