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. 2018 Aug 7;9:1789. doi: 10.3389/fmicb.2018.01789

FIGURE 4.

FIGURE 4

Relative survival of RR gene mutants and wild-type strain under multiple stresses. Stress tolerance of RR mutants was determined using exponentially growing cells. (A) Antioxidant reaction (0.125 mM H2O2); (B) heavy metal tolerance (12 μM CdSO4); (C) osmotic adaptation (300 mM NaCl); and (D) sodium dodecyl sulfate (SDS) tolerance (8 × 10-5g/ml). Bacterial survival is estimated as the ratio of the number of colonies obtained on NA (3.0 g/L beef extract; 5.0 g/L polypeptone; 1.0 g/L yeast extract; 10.0 g/L sucrose; 8.0 g/L agar pH7.0) with certain antibiotics and incubated for 48 h measured 16 h and none challenge. Relative survival is the ratio of mutant survival to that of the wild-type strain. Standard deviations of three experiments are shown. Significance was tested by Student’s T-test (∗ indicates significance at P < 0.05). Survival of mutants compared with that of wild-type; H, significantly increased; S, similar; and L, significantly decreased.