Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Genotype or description | Reference |
|---|---|---|
| Plasmids | ||
| pK18mob | Suicide vector to create a mutant by a single crossover; Kanr | Schäfer et al., 1994 |
| pK18mobsacB | Suicide vector to create mutant by double crossover recombination; Kanr | Schäfer et al., 1994 |
| pLAFRJ | Shuttle plasmid pLAFR3 derivate containing the multiple cloning sites of pUC19; Tcr | Jiang et al., 2009 |
| Strain | ||
| Xoc GX01 | Wild-type strain; Rifr | Lab collection |
| E. coli DH5α | Used for molecular cloning | Lab collection |
| NK0263–NK4109 (a total of 50 strains) | GX_XOC0263 to GX_XOC4109 (a total of 50 genes), mutants with insertions of response regulator genes of Xoc GX01, constructed by pK18mob vector integration; Kanr | This study |
| CNK2201 | Genetic complementary strain of NK2201 (mutant of GX_XOC2201); Rifr, Kanr, Tcr | This study |