Table 4.

Primer sequences (5′–3′) used to introduce site directed mutations in C3.

C3 mutation	Primer sequence (5′–3′)a
R102G	GTCAGAAAAGGGGGGCAACAAGTTCGTG
K155Q	CACCGTCAACCACCAGCTGCTACCC
L302P	GGGAGGTTGTGCCGAGCCGGAAGGTAC
P314L	GGTGCAGAACCTCCGAGCAGAAGAC
Y325H	GGGAAGTCTTTGCACGTGTCTGCCAC
V326A	GGAAGTCTTTGTACGCGTCTGCCACCGTC
S327P	GTCTTTGTACGTGCCTGCCACCGTCATC
V330I	CGTGTCTGCCACCATCATCTTGCACTC
K633R	CGGGCAGTGGGAGGGATTACGCCGG
R735W	CATCACAGAGCTGTGGCGGCAGCACGC
R1591G	CCCATCAAGTGCGGAGAAGCCCTGAAGC
G1606D	CTACCTCATGTGGGATCTCTCCTCCG
S1619R	GAGAAGCCCAACCTCCGCTACATCATCGG

^aNucleotides corresponding to the changed amino acid residue are underlined.