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. 2018 Aug 7;9:1828. doi: 10.3389/fmicb.2018.01828

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Copyright © 2018 Liu, Wang, Sun, Zhang, Zhang, Liu, Yu and Pan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sssfh1 is required for the full virulence of S. sclerotiorum. (A) The Sssfh1-silenced strains show the impaired capacity to invade soybean leaves. Mycelial plugs of the indicated strains were inoculated on soybean leaves and disease symptoms were observed and photographed at 3 dpi. This experiment was repeated three times. (B) Sssfh1-silenced strains caused reduced disease lesions on common bean leaves. Symptoms were photographically documented at 36 hpi from both adaxial (upper panel) and abaxial leaf epidermis (lower panel). (C,D) Quantitative analysis of the lesion sizes in (A,B). Data represent means ± SD from at least three independent experiments (one-way ANOVA, ^∗∗indicates significance at p < 0.01).