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. 2018 Aug 7;9:1828. doi: 10.3389/fmicb.2018.01828

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Copyright © 2018 Liu, Wang, Sun, Zhang, Zhang, Liu, Yu and Pan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SsSFH1 interacts with SsMSG5. (A) Yeast two-hybrid assay reveals that SsSFH1 interacts with SsMSG5 in AH109 yeast cells. Sssfh1 was inserted into pGBKT7 and the Ssmsg5, Ssfus3/Sskss1, and others were inserted into pGADT7. Co-transformed AH109 cells were streaked on the SD/-Leu-Trp or SD/-Leu-Trp-His-Ade with X-α-Gal. pGBKT7-Lam and pGADT7 were used as negative control, while pGBKT7-53 and pGADT7-T were used as positive control. pGBKT7-Sssfh1 and pGADT7 were used for self-activation detection. (B) The interaction between SsSFH1 and SsMSG5 was confirmed by BiFC assay in Arabidopsis protoplast. The well-known interaction proteins AtCRY2 and AtCIB1 were used as positive control, while the empty cYFP and SsSFH1-nYFP were used as negative control. One representative data is shown from at least three independent experiments.