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. 2018 Aug 7;9:1793. doi: 10.3389/fmicb.2018.01793

Figure 4.

Figure 4

Intergenic mutations that increased gene expression of the NADP(H) binding component of the insoluble pyridine nucleotide transhydrogenase in eGnd strains. (A) Network diagram of the insoluble (THD2pp) and soluble (NADTRHD) pyridine nucleotide transhydrogenase. THD2pp has been shown to provide an important source of NADPH in E. coli (Sauer et al., 2003). The insoluble pryidine nucleotide transhydrogenase is composed of two subunits encoded by pntA and pntB. The former contains the NADP(H) binding domain while the latter contains the proton pumping transmembrane domain (Johansson et al., 2005). (B) Operon diagram of pntA. Mutations that affected gene expression of pntA were found in all eGnd strains. The 1675937 SNP is 62 nucleotides downstream of the pntA transcription start site (TSS) and the 1676048 SNP is 49 nucleotides downstream of the pntA TSS. Mutations are annotated in red. (C) Mutation frequency and expression levels of pntA and ydgH. Note that all eGnd strains significantly elevated expression of pntA.