Fig. S5.

The anti-HCC ability and LRP6 interactions of SAMC precursor, S-allylcysteine (SAC), were weak. (A) Natural metabolic pathway from SAC to SAMC during garlic aging. (B) SAC slightly reduced cell viability of Hep3B and Huh-7 at 1 mmol/L without evident influence in human normal hepatocyte cell line LO-2 (n=4). (C) Change of migrated cell number after SAC treatment, examined by Transwell assay, in human hepatoma cell lines Hep3B and Huh-7 (n=4). (D) SPR and TSA analyses of the binding of SAC to recombinant human LRP6 protein. Data are presented in means±SEM. ^*P<0.05 between indicated group and control group.