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. 2017 Dec 10;8(4):602–614. doi: 10.1016/j.apsb.2017.10.004

Figure 3.

Fig. 3

Cellular uptake of Poly/PTX-SAs. (A) Quantitative cellular uptake evaluation. All the cells were treated with Poly SAs loaded with coumarin 6 instead of PTX in order to evaluate fluorescent uptake in cells. BC cells (500,000) were treated with Poly/coumarin 6-SAs (5 µg dye equivalent formulations) for 3 h. These cells were trypsinized, centrifuged and collected in 2 mL media, which were injected (50 µL cell suspension) into an Accuri C6 Flow Cytometer (Accuri Cytometer, Inc., Ann Arbor, MI, USA). Mean fluorescence intensity in FL1 channel (488 excitation, Blue laser, 530 ± 15 nm, FITC/GFP) was measured. Data represented as mean ± standard error of the mean (n = 3), *P<0.05. (B) Qualitative internalization efficiency of Poly/coumarin 6-SAs in BC cells. In above treatment condition, uptake of Poly/couamrin 6-SAs in BC cells was viewed using EVOS® FL Imaging System (AMF4300, Life Technologies, Carlsbad, CA, USA). Bar equals to 200 μm. This study suggests fluorescence intensity in the order: PVP>PVA>PMEAVH>Povacoat F> PA-HCL>PEI>Poloxamer 188>PAA coumarin 6-SAs.