Figure 4.

Self-assembly formation of PVP/PTX-SAs. A) Self-assembly formation was confirmed by binding potential (quenching) of PTX conjugated dye (PTX, Oregon Green 488 conjugate (Flutax-2, Molecular Probes Inc., Life Technology, OR, USA)) with PVP solution (5–80 µg/mL). Fluorescence was studied by exciting at 496 nm and emission between 450–600 nm. (B) FTIR spectra of PVP/PTX-SAs, PTX, and PVP. The presence of 1033 cm^–1 (characteristic PTX peak) and 1270, 1284, 1420 and 1654 cm^–1 characteristic PVP peak) confirms the presence of both PVP and PTX in the self-assembly formation of PVP/PTX-SAs. (C) Schematic and hypothetical structural presentation of PVP/PTX-SAs. Color, dimensions, assembly are not comparable to synthesized formulation. Morphology of PVP/PTX-SAs imaged by using AMT camera at a direct magnification of 100,000× under the TEM on a 150 mesh standard TEM grid, stained by 2% w/v uranyl acetate solution. The average particle size viewed under TEM 53.81±2.6 nm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)