Table 3.

Operating conditions of HPLC for method development.

Analytes	Aldicarb; Atrazine; Carbofuran; Carbendazim; Methoxychlor; Parathion methyl; Spiromesifen
Gradient elution	Solvent B: 0.01 min. 55% B; 5.00 min. 30% B; 7.50 min. 20 % B; 9.00 min. 25% B; 14.01 min. 35% B; 18.01 min. Stop
Total run time	18.01 min.
Column	C18 column
Column Temperature	38 °C
Flow rate	1 mL/min
Injection volume	12 μL
Detection wavelength	258 nm

Where A is Acetonitrile; B is water.