FIGURE 4.

Incubation of IV particles with Oximacro^® abrogates infectivity. IAV or IBV (2 × 10⁴ PFU) were incubated at 37°C (A) or at 4°C (B) for various lengths of time in the absence of Oximacro^® (NT) (closed circles) or with 25 g of the Oximacro^® (closed squares). After incubation, the samples were diluted to reduce Oximacro^® concentration below that which inhibits IV attachment (0.25 μg/ml). Plaques were microscopically counted, and the mean plaque counts was expressed as PFU/ml on a log10 scale. The data shown represent means ± SD (error bars) of three independent experiments performed in duplicate. ^∗∗∗p < 0.001, ^∗∗p < 0.01, and ^∗p < 0.05 compared with the titer of untreated controls (NT).