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A: All the nuclei were uniformly weakly stained in control group; B: RGC-5 cells exposed to 20 ng/mL IL-8 for 24h. Apoptotic cells were identified by condensation of chromatin and fragmentation of their nuclei; C: RGC-5 cells exposed to 20 ng/mL IL-8 and pre-treatment with G31P for 24h. The amount of apoptotic cells was decreased with G31P pre-incubation. Three experiments were performed with similar results.
