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. 2018 Aug 7;9:1678. doi: 10.3389/fmicb.2018.01678

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Copyright © 2018 Chen, Sun, Yang, Song and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro activity of the phage against the wild-type and capsule-deficient mutant strains. (A) In vitro activity of the phage against wild-type P. multocida strain HB03. The phage was mixed with the bacteria at a MOI of 10 or 100 and then cultured at 37°C for 30 min () or 60 min (). Results show the number of viable bacteria following incubation. Experimental data were analyzed by two-way analysis of variance and are expressed as the mean ± SD. The viable cell counts in each of the treatment groups were compared with that of the control, with significant differences indicated by ^∗P < 0.05; ^∗∗P < 0.01; and ^∗∗∗P < 0.001. (B) In vitro activity of the phage against the capsule-deficient P. multocida strain. Conditions and data interpretation were the same as described in (A).

Inline graphic — In vitro activity of the phage against the wild-type and capsule-deficient mutant strains. (A) In vitro activity of the phage against wild-type P. multocida strain HB03. The phage was mixed with the bacteria at a MOI of 10 or 100 and then cultured at 37°C for 30 min () or 60 min (). Results show the number of viable bacteria following incubation. Experimental data were analyzed by two-way analysis of variance and are expressed as the mean ± SD. The viable cell counts in each of the treatment groups were compared with that of the control, with significant differences indicated by ^∗P < 0.05; ^∗∗P < 0.01; and ^∗∗∗P < 0.001. (B) In vitro activity of the phage against the capsule-deficient P. multocida strain. Conditions and data interpretation were the same as described in (A).