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. 2018 Aug 7;9:1678. doi: 10.3389/fmicb.2018.01678

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Copyright © 2018 Chen, Sun, Yang, Song and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Serum sensitivity assay. (A) P. multocida strain HB03 mixed with depolymerase was incubated with mouse serum or whole-blood. (B) P. multocida strain HB03 with depolymerase was incubated with human serum. The mixtures were incubated at 37°C for 2 h. The number of viable bacteria was determined by plating and enumeration of serial dilutions of the suspension. Assays were repeated at least three times. Experimental data were analyzed by one-way analysis of variance and are expressed as the mean ± SD. The viable cell counts in each of the treatment groups were compared with that of the PBS control, with significant differences indicated by ^∗P < 0.05; ^∗∗P < 0.01; and ^∗∗∗P < 0.001.