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. 2018 Jul 31;5:74. doi: 10.3389/fmolb.2018.00074

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Copyright © 2018 Sgro and Costa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Different designs of a TEM (transmission electron microscopy) grid and semi-automated method for specimen vitrification. (A) Examples of a TEM grid with irregular hole size foil (Lacey) or with defined hole diameter and spacing (Quantifoil). (B) An automated plunge-freezing device is commonly used for specimen vitrification. Sample is applied with a pipette at the surface of the cryo-EM grid and sample excess is removed by blotting with filter paper, followed by immediate freezing in liquid ethane. The specimen can be frozen on a grid with (i) or without (ii) a thin continuous film made of different materials. TEM grids with different grid mesh, foil and grid support materials can be used during specimen freezing.