Fig. 2.

The S1PR1 antagonist VPC44116 inhibits FTY720- and CYM-5442–mediated antinociception in the tail–flick test. Mice were injected i.c.v. with vehicle or VPC44116 (25 µg), followed 10 minutes later by injection (i.p.) of FTY720 (6 mg/kg) (A) or CYM5442 (10 mg/kg) (B). Radiant heat tail–flick latencies were determined 15 (A) or 30 (B) minutes after i.p. injection. Baseline latencies were 3.97 ± 0.26 seconds (A) and 3.99 ± 0.31 seconds (B). Data are expressed as mean maximum possible effect (%MPE) ± S.E.M. (n = 5 mice/group). *^,***P < 0.05, 0.001 different from agonist alone; ^#P < 0.05 different from VPC44116 alone.