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S1P-stimulated [35S]GTPγS binding is decreased in the spinal cord of repeated FTY720-treated mice. Mice were treated with FTY720 (2 mg/kg, twice per day) or vehicle for 6.5 days, and S1P-stimulated [35S]GTPγS binding was determined using the indicated concentrations of S1P. Data are expressed as mean percentage of stimulation ± S.E.M. (n = 3 mice/group).
