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. 2018 Aug 7;9:997. doi: 10.3389/fpls.2018.00997

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Copyright © 2018 Gao, Wang, Xu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Molecular characterization of transgenic wheat. (A) Schematic diagram of the transformation vector pMWB122-TaWRKY2. (B) PCR analysis of the three transgenic lines from T₁ to T₃. (C) Relative expression of TaWRKY2 in transgenic wheat. (D) Southern blot analysis of HindIII digested genomic DNAs from untransformed and T₃ transgenic plants. WT, wild type; P, plasmid. Asterisks represent statistically significant difference compared WT (Student’s t-test, ^∗P < 0.05, ^∗∗P < 0.01).