Table 2.

Regulation of mitochondrial bioenergetics in the heart by SIRT3.

SIRT3 targets	Effect of deacetylation	Confirmed by in vitro deacetylase assays	Biological function	Reference
Acetyl-CoA synthetase 2	↑	Yes	Acetate metabolism	Hallows et al., 2006; Schwer et al., 2006
Glutamate dehydrogenase	↑	Yes	Amino acid catabolism	Lombard et al., 2007; Schlicker et al., 2008
FOXO3a	↑	Yes	Transcription	Sundaresan et al., 2009
LCAD	↑	Yes	Fatty acid metabolism	Hirschey et al., 2010; Chen et al., 2015; Koentges et al., 2015
PDH	↑	Yes	TCA cycle	Mori et al., 2013; Ozden et al., 2014; Zhang et al., 2018
Aconitase	↓	No	TCA cycle	Fernandes et al., 2015
IDH	↑ ↓ (?)	Yes	TCA cycle; antioxidant system	Schlicker et al., 2008; Nadtochiy et al., 2017
MDH	N.C.	Yes	TCA Cycle	Nadtochiy et al., 2017
Complex I	↑/N.C. (?)	Yes	ETC	Ahn et al., 2008; Porter et al., 2014; Koentges et al., 2015, 2016; Parodi-Rullán et al., 2017
Complex II	↑	Yes	ETC	Finley et al., 2011; Parodi-Rullán et al., 2017
Complex III	N.C. (?)	No	ETC	Koentges et al., 2015; Parodi-Rullán et al., 2017
Complex IV	N.C. (?)	No	ETC	Koentges et al., 2015; Parodi-Rullán et al., 2017
Complex V	↑	No	OXPHOS	Pillai et al., 2015
Oxoguanine glycosylase	↑	No	BER	Pillai et al., 2016, 2017
MnSOD (SOD2)	↑/N.C.	Yes	Mitochondrial antioxidant system	Sundaresan et al., 2009; Tao et al., 2010; Porter et al., 2014; Pillai et al., 2015, 2017; Yang et al., 2016b; Parodi-Rullán et al., 2017
Mitochondrial ribosomal protein L10 (MRPL10)	↓	Yes	Protein synthesis	Yang et al., 2010
Liver kinase B1 (LKB1)	?	Yes	AMPK Pathway	Pillai et al., 2010
Ku70	?	Yes	DNA repair	Sundaresan et al., 2008

N.C., no change. cccPurified protein only (not in the heart).