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. 2018 Aug 14;6(15):e13820. doi: 10.14814/phy2.13820

Figure 4.

Figure 4

(A) Representative western immunoblots of eNOS, Ser1177 p‐eNOS, iNOS, Cox1, Cox2, Cav‐1, and actin in lipid raft fractions from WT LF aorta under basal conditions and following relaxation to ACh (1 μmol/L). Only eNOS was localized to low‐density cav‐1 containing fractions under basal conditions which shifted to high‐density fractions in ACh‐stimulated aorta. Expression of eNOS (B), iNOS (C), Cox1 (D), Cox 2 (E), Cav‐1 (F), and actin (G) as a percent of total in low‐density fractions (1) to heavy density fractions (9) of aorta from WT mice on LF diet under basal conditions or following relaxation to acetylcholine (ACh, 1 μmol/L). eNOS was primarily localized to cav‐1 containing low‐density fractions under basal conditions which shifted to heavy fractions with ACh. iNOS, Cox1, and Cox2 were not localized to low‐density cav‐1 containing fractions or affected by ACh. The percent of actin in heavy density fractions increased with ACh (G). Mean ± SEM. Two‐tailed unpaired t test.