Figure 3.

Effects of NG (5.5 mM) and HG (35.5 mM) on the protein expression levels and secretion of TGF-β1 and TGF-β2 in human lens epithelial B3 cells at 0, 3, 6 and 12 h. Protein expression levels of (A) TGF-β1 and (B) TGF-β2 were determined using western blot analysis. The secretion of (C) TGF-β1 and (D) TGF-β2 was determined using ELISA. Data are presented as the mean ± standard deviation. *P<0.05 vs. 0 h in the same group; ^#P<0.05 vs. NG group at the same time point. NG, normal glucose; HG, high glucose; TGF-β, transforming growth factor-β.