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. 2018 Jun 13;16(2):1417–1425. doi: 10.3892/etm.2018.6301

Figure 2.

Figure 2.

SIRT5 suppresses insulin secretion and pancreatic β-cell proliferation. MIN6 and INS-1 cells were transfected with an empty plasmid vector, a plasmid vector containing SIRT5, SCR or siSIRT5. At 48 h following transfection, the (A) mRNA levels of SIRT5 were detected by reverse transcription quantitative polymerase chain reaction analysis and (B) SIRT5 protein levels were detected by western blotting. (C) A colony formation assay and (D) a CCK-8 assay was performed to assess the effect of SIRT5 on cell proliferation. Each experiment was repeated three times. (E) Transfected MIN6 and INS-1 cells were also incubated with 1 mM or 20 mM glucose for 90 min and the rate of insulin secretion was detected in each group. Each experiment was repeated three times. The results are presented as the mean ± standard deviation. *P<0.05 vs. SCR and #P<0.05 vs. vector. SIRT5, sirtuin 5; Vector, pcDNA3.1 plasmid vector; SCR, scrambled small interfering RNA; siSIRT5, small interfering RNA targeting sirtuin 5; OD, optical density.