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. 2018 Jun 19;16(2):1241–1249. doi: 10.3892/etm.2018.6324

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Copyright: © Chira et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Evaluation of senescence in DLD-1 colorectal cell line exposed to a low concentration of deuterium. Cells were cultivated for 13 passages in RPMI-1640 medium in SC or low deuterium, and assessed for expression of β-galactosidase (red arrows), which is present in senescent cells only. A slight activation of senescence was observed in cells cultivated in DDW growth medium. DDW, deuterium-depleted water.