Table I.
Primers used in reverse transcription-quantitative polymerase chain reaction experiments.
| Genes | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| GAPDH | GGGCTCATGACCACAGTCCATGC | CCTTGCCCACAGCCTTGGCA |
| Col X | ACGCATCTCCCAGCACCAGAATC | GGGGCTAGCAAGTGGGCCCT |
| Runx2 | GGTTGTAGCCCTCGGAGAGG | GCCATGACGGTAACCACAGTC |
| Smad1 | AAAGACCTGTGGCTTCCGTCT | TTATCGTGGCTCCTTCGTCAG |
| Smad2 | ATGTCGTCCATCTTGCCATTC | AACCGTCCTGTTTTCTTTAGCTT |
| Smad3 | GCTGCCCTCCTAGCTCAG | GGTGCTGGTCACTGTCTGTC |
| Smad4 | GAGAACATTGGATGGACGACT | CACAGACGGGCATAGATCAC |
| Col2a1 | CCAGCTGACCTCGCCACTGC | GGGTCCAGGCGCACCCTTTT |
| MMP10 | GCAGCCCATGAACTTGGCCACT | AGGGACCGGCTCCATACAGGG |
| Vcam1 | GATAGACAGCCCACTAAACGCG | GAATCTCTGGATCCTTGGGG |
Col X, type X collagen; Runx2, runt-related transcription factor 2; Smad, mothers against decapentaplegic homolog; Col 2a, collagen, type II, α; MMP10, matrix metalloproteinase 10; Vcam1, vascular cell adhesion molecule 1.