Figure 1.

Reverse transcription polymerase chain reaction performed on 100 MII-stage mouse oocytes with primers specific for Epac1 (product length, 116 bp), Rap1 (product length, 95 bp) and GAPDH (product length, 98 bp). Lanes: M, 100-bp DNA ladder; 1–3, amplification products for Epac1, Rap1 and GAPDH, respectively; 4–6, negative controls without reverse transcriptase, RNA and complementary DNA in the reaction tube, respectively. Rap1, Ras-related protein-1; Epac, exchange proteins directly activated by cyclic adenosine monophosphate.