FIGURE 4.

Effect of TRPC1 or IP3R inhibitors, combined with TMZ, on the proliferation of U251 cells. (A) Cells were incubated for 20 min and MTT analysis was performed in the absence of any drug (Control) or in the presence of 5 μM TMZ. In some experiments, 2 μg/mL TRPC1 neutralizing antibody (TRPCi) and 1 μM Nifedipine (Nife) were added 20 min before the addition of TMZ. (B) Cells were incubated for 20 min, and MTT analysis was performed in the absence of any drug (Control) or in the presence of 5 μM TMZ. In some experiments, 1 μM Xestspongin C (Xest) and 1 μM Ryanodine (Ryan) were added 20 min prior to the addition of TMZ. Data are presented as the mean ± SEM of six replicates. ^∗Indicates a statistically significant (P < 0.05) difference from the negative control group.