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. 2018 May 30;16(2):537–546. doi: 10.3892/etm.2018.6234

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Copyright: © Ding et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Expression and distribution of NRP-1 in human gastric cancer cells. (A) Levels of NRP-1 protein expression in human gastric cancer cell lines (BGC-823, SGC-7901 and MKN-74) measured by western blot analysis. β-actin was used as an internal control. (B) Levels of NRP-1 mRNA in BGC-823 cells measured by reverse transcription-quantitative polymerase chain reaction. (C) The distribution of NRP-1 proteins in BGC-823 cells measured by confocal fluorescence microscopy. Hoechst staining (blue) indicates cell nuclei and TRITC staining (red) indicates NRP-1 expression. Magnification, ×400. Scale bar, 20 µm. NRP-1, neuropilin 1; b1/b2, coagulation factor V/VIII homology-like domains; mAb, monoclonal antibody; BGC-823, SGC-7901 and MKN-74, human gastric cancer cell lines; TRITC, tetramethylrhodamine.