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. 2018 Aug 7;6:86. doi: 10.3389/fcell.2018.00086

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Copyright © 2018 Ito, Kasai, Kumagai, Suzuki, Ochiai-Noguchi, Irikura, Miyake and Murakami.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Surface plasmon resonance imaging analysis of the interactions between CADM1 and its binding partners. (A) SPRi analysis was performed by injecting 2.4 μM CADM1-Fc for 4 min onto the sensor chip where the 10 IgSF-Fc proteins were spotted. The analysis was performed in hexaplicate and the averaged signal of the six spots are shown. Only five molecules giving positive signals are shown. (B) The percent change in reflectivity (%ΔR) at 60 s was visualized as a bright spot on the image.