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. 2018 Jun 12;16(2):1225–1231. doi: 10.3892/etm.2018.6288

Figure 3.

Figure 3.

Overexpression of miR-423-5p promotes apoptosis and upregulates caspase expression in colon cancer cells. (A) Flow cytometric analysis of cell apoptosis by Annexin V and PI staining in SW620 and HCT116 cells transfected with miR-NC and miR-423-5p. n=3. (B) Western blot analysis of caspases 3, 8 and 9 and p53 expression in HCT116 and SW620 cells transfected with miR-NC and miR-423-5p. GAPDH was used as a loading control. n=3. Values are expressed as the mean ± standard deviation. **P<0.01 vs. miR-NC group. miR-423-5p, microRNA-423-5p; PI, propidium iodide; FITC, fluorescein isothiocyanate; NC, negative control.