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. 2018 Jun 8;16(2):730–738. doi: 10.3892/etm.2018.6274

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Inhibitory effects of anti-CYR-61 on H358 cells in vitro. (A) MTT assays were used to analyze the inhibitory effects of anti-CYR-61 on H358 cell viability. (B) Immunofluorescence (using Alexa Fluro^® conjugated Goat anti-rabbit Immunoglobulin G) was used to detect CYR-61 expression after anti-CYR-61 treatment. (C) Migration analysis was performed to determine the effect of anti-CYR-61 on H358 cell migration. (D) Invasion analysis was performed to determine the effect of anti-CYR-61 on H358 cell invasion. C and D were stained using hematoxylin and eosin. Data was presented as the mean ± standard deviation of triplicate samples. **P<0.01 vs. the control. CYR-61, cysteine-rich angiogenic inducer-61.