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Maximum likelihood tree based on a 430-bp fragment of the NS5 gene of Powassan (lineage I) and deer tick virus (lineage II). The phylogenetic tree was produced with 100 bootstrap replications and branch length is proportional to genetic distance using BioNJ algorithm analyses. Louping ill virus (KF056331), West Nile virus (DQ164202), and Dengue virus (KX274130) were used as outgroups. The ruler shows the branch length for a pairwise distance equal to 0.05.
