Mean vector competence of Argentine mosquitoes across days 5, 10, 14, and 21 held at either temperature cycles mimicking temperature at sites during trapping (site temp) or the mean hourly temperature cycle across sites (mean temp). Significant differences were measured for overall infection and dissemination rates for both viruses and all populations (Fisher’s exact test, P < 0.05), with the exception of chikungunya virus (CHIKV) in Salta mosquitoes, for which no effect of temperature was measured.