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The TUG1 siRNA transfected Saos-2 and U2OS cells were then transfected with NC inhibitor or miR-212-3p in, respectively. (A and B) Following transfection, a Transwell assay was used to study cell invasion. (C and D) Western blotting was used to examine the protein expression of MMP2 and MMP9. **P<0.01 vs. siTUG1+NC in. TUG1, taurine upregulated gene 1; siRNA, short interfering RNA; NC, negative control; miR, microRNA; in, inhibitor; MMP, matrix metalloproteinase.
