Figure 4.

DCUN1D1 expression is negatively regulated by miR-302-3p in HeLa cells. The (A) mRNA and (B) protein expression levels of DCUN1D1 were examined in HeLa cells transfected with miR-302-3p mimic or scramble miR mimic (NC), respectively. Then, HeLa cells were transfected with NC inhibitor or miR-302-3p inhibitor. (C) After transfection, quantitative real-time PCR was used to evaluate the miR-302-3p expression level. The (D) mRNA and (E) protein expression levels of DCUN1D1 were also evaluated. **P<0.01 vs. NC; *P<0.01 vs. miR-302-3p inhibitor. DCUN1D1, defective in cullin neddylation 1 domain containing 1; NC, negative control.