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. 2018 May 29;16(2):695–703. doi: 10.3892/etm.2018.6225

Figure 3.

Figure 3.

Knockdown of MIST1 and SNAI1 increases adhesion and induces cells to undergo anoikis. (A) MIST1 was knocked down in the A375 and MV3 cell lines, as confirmed by western blot analysis. (B) Knockdown of MIST1 improved the adhesion ability of A375 cells (scale bar, 200 µm). Findings for MV3 were similar, data not shown. (C) Inhibition of MIST1 decreased the viability of A375 cells in suspension culture, indicating increased anoikis. Findings for MV3 were similar, data not shown. (D) SNAI1 was knocked down in the A375 and MV3 cell lines, as confirmed by western blot analysis. (E) Knockdown of SNAI1 improved the adhesion ability of A375 cells (scale bar, 200 µm). (F) Inhibition of SNAI1 decreased the viability of A375 cells in suspension culture, indicating increased anoikis. NS, no significance; *P<0.05 vs. control. shMIST1, small hairpin RNA targeting muscle intestine and stomach expression 1; Con, control.