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. 2018 May 29;16(2):695–703. doi: 10.3892/etm.2018.6225

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Copyright: © Lee et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — MIST1 promotes SNAI1 transcription by directly binding to its promoter region. (A) MIST1 binding motif. (B) Design of 7 chromatin regions of the SNAI1 promoter containing the MIST1 binding motif for ChIP. (C and D) ChIP performed on HUVEC and NHEM 2493 cells demonstrated that MIST1 was enriched in samples containing region 5 of the SNAI1 promoter. (E) A luciferase reporter assay determined that the −1,199 to −753 bp segment upstream of TSS site of SNAI1 combined with MIST1. TSS, transcription start site; Ctrl, control; IgG, immunoglobulin G; ChIP, chromatin immunoprecipitation; *P<0.05 vs. control; MIST1, muscle intestine and stomach expression 1; HUVEC, human umbilical vein endothelial cells; NHEM 2493, normal human epidermal melanocytes.