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. 2018 Jun 13;16(2):1095–1102. doi: 10.3892/etm.2018.6298

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Copyright: © Cai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Silencing IGFBP7 inhibited apoptosis of podocytes mediated by HG. Podocytes were treated with PBS (control), siNC, 60 mM glucose (HG), siNC and HG, and siIGFBP7 and HG for 12 h. (A) Total apoptotic cells were measured using flow cytometry in treated podocytes. (B) Quantitative analysis of number of apoptotic cells. (C) AIF, caspase-3 and caspase-9 expression was detected via reverse transcription-quantitative polymerase chain reaction. (D) Western blotting of AIF, c-caspase-3 and c-caspase-9. Protein levels were quantified using densitometry. ***P<0.001. IGFBP7, insulin-like growth factor-binding protein 7; HG, high glucose; siNC, normal control small interfering RNA; siIGFBP7, IGFBP7 small interfering RNA; AIF, apoptosis inducing factor; c, cleaved; PI, propidium iodide; FITC, fluorescein isothiocyanate.