Figure 4.

Silencing IGFBP7 inhibited epithelial mesenchymal transformation of podocytes induced by HG. Podocytes were treated with PBS (control), siNC, 60 mM glucose (HG), siNC and HG, and siIGFBP7 and HG for 12 h. (A) Reverse transcription-quantitative polymerase chain reaction was used to detect nephrin, p-cadherin, FSP-1, α-SMA and Snail expression in treated podocytes. Nephrin, p-cadherin, FSP-1, α-SMA, and Snail expressions were analyzed via (B) western blotting and (C) quantified. **P<0.01; ***P<0.001. IGFBP7, insulin-like growth factor-binding protein 7; HG, high glucose; siNC, normal control small interfering RNA; siIGFBP7, IGFBP7 small interfering RNA; p, phosphorylated; FSP-1, fibroblast-specific protein-1; α-SMA, α-smooth muscle actin.