Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 13;16(2):1095–1102. doi: 10.3892/etm.2018.6298

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Cai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 5. — Silencing IGFBP7 suppressed the TGF-β1/Smad pathway in podocytes induced by HG. Podocytes were treated with PBS (control), siNC, 60 mM glucose (HG), siNC and HG, and siIGFBP7 and HG for 12 h. (A) TGF-β1 concentration was detected using ELISA. (B) The mRNA expression levels of TGF-β1 and Smad7 were analyzed using reverse transcription-quantitative polymerase chain reaction. (C) The protein expression levels of TGF-β1 and Smad7 were analyzed via western blotting. (D) The protein expression levels of p-Smad3 and Smad3 were analyzed via western blotting. *P<0.05; ***P<0.001. IGFBP7, insulin-like growth factor-binding protein 7; TGF-Smad7 were analyzed via western bloSmad, mothers against decapentaplegic homolog; HG, high glucose; siNC, normal control small interfering RNA; siIGFBP7, IGFBP7 small interfering RNA; p, phosphorylated.