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. Author manuscript; available in PMC: 2019 May 3.
Published in final edited form as: Cell. 2018 May 3;173(4):1031–1044.e13. doi: 10.1016/j.cell.2018.03.062

Figure 3. Retained and high splicing intermediate introns.

Figure 3

(A) Scatterplot of the number of exon junction reads verses number of exon-intron boundary reads from WT RNA-Seq data for all S. pombe introns colored by type as indicated. Introns previously mentioned in Figure 2 are labeled.

(B) Violin plots comparing percent of intron retention between intron groups defined in A. Previously annotated introns not covered by 5′-P or 3′-OH data are shown in white.

(C) Examples of introns with different levels of splicing intermediates. Numbers in brackets: 3′-OH 3′ read ends terminating at the 5′SS or 3′SS.

(D) Histogram of percent of splicing intermediate (calculated as fraction of 5′SS 3′-OH to all intron 3′-OH reads) across all introns with ≥ 25 3′SS 3′-OH reads in both replicates. Dashed vertical lines indicate introns shown in A.