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. Author manuscript; available in PMC: 2019 Jul 25.
Published in final edited form as: Neuron. 2018 Jun 28;99(2):315–328.e5. doi: 10.1016/j.neuron.2018.06.010

Figure 4.

Figure 4

Glutamate deactivation time courses of diheteromeric and triheteromeric NMDARs. A. Normalized representative current responses to brief (<5 ms) application of 1 mM glutamate in the continuous presence of 100 μM glycine from outside-out patches excised from oocytes expressing of GluN1/GluN2A/GluN2A (blue), GluN1/GluN2AC1/GluN2CC2 (red), or GluN1/GluN2C/GluN2C (green). B. Deactivation time constants for GluN1/GluN2A/GluN2A and GluN1/GluN2C/GluN2C receptors were obtained by fitting the time course with a single exponential function. Deactivation time constants for GluN1/GluN2AC1/GluN2CC2 receptors were obtained by fitting the response time course with the sum of three exponential functions. C. The total response from GluN1/GluN2AC1/GluN2CC2 receptors is shown with the GluN1/GluN2A/GluN2A and GluN1/GluN2C/GluN2C receptor components, which were simulated using the deactivation time constant determined from oocytes expressing GluN1/GluN2A/GluN2A and GluN1/GluN2C/GluN2C receptors. The amplitudes of the diheteromeric contribution to the time course were set as the escape current measured from GluN1/GluN2AC1/GluN2AC1 and GluN1/GluN2CC2/GluN2CC2 receptors. The GluN1/GluN2AC1/GluN2CC2 receptor component was isolated by subtracting the GluN1/GluN2A/GluN2A and GluN1/GluN2C/GluN2C receptor components from the total current. D. Summary of deactivation time constants for the GluN1/GluN2AC1/GluN2CC2 receptor component determined from 4 different batches of oocytes.