Table 2.
Single channel properties for GluN1/GluN2A/GluN2C
| Open duration | GluN2AC1/GluN2AC2 | GluN2CC1/GluN2CC2 | GluN2AC1/GluN2CC2 |
|---|---|---|---|
| Tau 1, ms (area) | 0.037±0.004 (23.7±0.8 %) | 0.034±0.005 (15.1±2.8 %) | 0.084±0.05 ms (29.4±3.5 %) |
| Tau 2, ms (area) | 0.303±0.03 (7.3±1.7%) | 0.406±0.05 (49.1±5.5%) | 0.547±0.12 (26.8±10.6%) |
| Tau 3, ms (area) | 3.35±0.69 (68.9±2.2%) | 1.02±0.11 (35.6±6.6%) | 1.63±0.25 (43.7±10.7%) |
| Mean open time, ms | 2.83±0.56 | 0.70±0.055* | 0.84±0.070* |
| Overall Open probability | 0.17±0.065 | 0.049±0.025* | 0.015±0.0092* |
| Intervals | 57,369 | 72,210 | 18,824 |
| Closed duration | GluN2AC1/GluN2AC2 | GluN2CC1/GluN2CC2 | GluN2AC1/GluN2CC2 |
|
| |||
| Tau 1, ms (area) | 0.026±0.0 (35.9±1.6 %) | 0.024±0.002 (26.6±3.5 %) | 0.027±0.003 ms (30.8±4.5 %) |
| Tau 2, ms (area) | 0.212±0.006 (22.4±1.2%) | 0.24±0.02 (10.3±1.3 %) | 0.26±0.04 (14.4±0.78 %) |
| Tau 3, ms (area) | 0.96±0.05 (16.9±0.8%) | 2.89±0.58 (19.1±2.0%) | 3.29±0.99 (19.3±3.3%) |
| Tau 4, ms (area) | 3.39±0.40 (21.1±1.6%) | 11.9±1.5 (29.1±5.3%) | 12.8±4.7 (27.9±3.6%) |
| Tau 5, ms (area) | 8.24±2.2 (3.1±0.5%) | 47.8±8.8 (13.4±3.3%) | 61.2±25.4 (4.8±1.4%) |
| Tau 6, ms (area) | 1754±485 (0.6±0.2%) | 349±164 (1.6±0.7%) | 2853±399 (2.8±0.9%) |
| Intervals | 59,449 | 71,410 | 18,678 |
| Burst Properties | GluN2AC1/GluN2AC2 | GluN2CC1/GluN2CC2 | GluN2AC1/GluN2CC2 |
|
| |||
| Burst length, ms | 654±95 | ND | 209±36 |
| Burst Open Probability | 0.60 | ND | 0.15 |
| Opens in Burst | 99.9% | ND | 99.2% |
| Number Opens/Burst | 504±139 | ND | 64±17 |
| Number of Bursts | 309 | ND | 967 |
| Tcrit (ms) | 41±6.2 | ND | 127±15 |
| Chord conductance | GluN2AC1/GluN2AC2 | GluN2CC1/GluN2CC2 | GluN2AC1/GluN2CC2 |
|
| |||
| γHIGH (area) | 75±5.5 pS (92±0.4%) | – | 75±3.0 pS (29±1.2%) |
| γ MEDIUM (area) | 58±5.0 pS (8±0.4%) | 47±1.8 pS (78±2.2%) * | 59±2.3 pS (66±1.2%) |
| γ LOW (area) | – | 29 ±1.7 pS (22±2.2%) * | 44±1.2 pS (5±0.05%) * |
| Number of currents | 104,014 | 83,536 | 20,116 |
| Number of patches | 3 | 7 | 7 |
Values are mean+SEM. Time constants and relative areas for maximum likelihood fits of the open and shut duration distributions to the sum or 3 to 6 exponential functions. Chord conductances were calculated assuming a reversal potential of 0 mV. Burst properties were calculated using a Tcrit determined for each patch to optimally separate the two slowest components of the shut duration histogram. ND indicates not determined.
*
p<0.05, significantly different from GluN1/GluN2AC1/GluN2AC2, ANOVA, Tukey post hoc, Holm-Bonferroni correction for family wise error. Power to detect an effect size of 1 was 0.9.