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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Acta Biomater. 2018 Jun 14;76:126–134. doi: 10.1016/j.actbio.2018.06.021

Figure 4.

Figure 4.

Migratory and proliferating cells in injured meniscus explants.

DAPI, β–actin and Ki67 stained sections of explants cultured for 2 weeks (n=3-6 per group).

a-d. Injured meniscus cultured without DMS: (a) Merged; (b) DAPI; (c) β–actin; (d) Ki67.

e-h. Injured meniscus cultured with DMS: (e) Merged; (f) DAPI; (g) β–actin (h) Ki67.

i-l. Injured meniscus cultured with DMS-Hep-PDGF-BB: (i) Merged; (j) DAPI; (k) β–actin; (1) Ki67.

m. β–actin staining of injured meniscus cultured with DMS-Hep-PDGF-BB. The elongated actin subunits (lamellipodia) are strongly stained lamella and are indicative of cell migration. Non-migrating cells show negative actin staining or short actin structures. The area marked by a yellow square in panel k is shown at higher magnification in panel m.

n. Graph with % lamellipodium positive cells.

o. Graph with the ratio of cell diameter (long/short diameter).

p. Graph with Ki67 positive cells.

a-l: 20x; m: 60x