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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Auton Neurosci. 2018 Jun 8;213:51–59. doi: 10.1016/j.autneu.2018.05.009

Figure 3. Impact of S. Typhimurium challenge on ileum mucosa cholinergic receptor gene expression.

Figure 3.

Salmonella Typhimurium enhances muscarinic receptor mRNA gene expression. mRNA gene expression was quantified utilizing two step reverse transcriptase quantitative PCR (RT-qPCR) for cholinergic muscarinic receptors 1, 2, 3 and cholinergic nicotinic receptor alpha 7 subunit (CHRM1, CHRM2, CHRM3, and CHRNA7 respectively). The data are presented as 2-ΔΔCT in gene expression relative to control group, normalized to beta-actin (ACTB) before statistical analysis. n = 6 controls and 6 challenged per bar. Student’s t-test compared controls vs S. Typhimurium challenge of each cholinergic receptor. * = p<0.05.