Fig. 2.

Silencing of GNB4 in 182^R-6 and TAM^R-1 cells via DNMT3B. a, Total RNA isolated from S05, 182^R-6, and TAM^R-1 cells was subjected to qRT-PCR using a primer set specific to GNB4. Whole cellular lysate was prepared from S05, 182^R-6, and TAM^R-1 cells, and Western blot analysis was performed using an antibody against GNB4. b, Whole cellular lysate was prepared from S05, 182^R-6, and TAM^R-1 cells, and Western blot analysis was performed using antibodies against DNMT1, DNMT3A, DNMT3B, and MeCP2. c, 182^R-6 and TAM^R-1 cells were transiently transfected with either 200 nM DNMT3B siRNA or 200 nM negative control siRNA; 72 h after transfection, total RNA isolated from these cells was subjected to qRT-PCR using a primer set specific to GNB4. d, Seventy-two hours after transfection, whole cellular lysate prepared from 182^R-6 and TAM^R-1 cells was transfected with either 200 nM DNMT3B siRNA or 200 nM negative control siRNA, and was subjected to Western blot analysis using antibodies against DNMT1, DNMT3A, DNMT3B and GNB4. Asterisk indicates p < 0.03