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. 2018 Aug 13;18:139. doi: 10.1186/s12903-018-0598-z

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a Peripheral blood after centrifugation: red blood cells at the bottom, PRF in the middle, and platelet-poor plasma at the top. b PRF clot. c PRF membrane. d An periapical radiograph of #45 with apical periodontitis in a 12-year-old girl. The case was treated by RET+PRF in #45. e Three-month follow-up periapical radiograph of tooth #45. f Six-month follow-up radiograph. g Nine-month follow-up radiograph. h Twelve-month follow-up radiograph showing complete periapical radiolucency resolution, root apex closure, root elongation and root canal wall thickening